ABSTRACT
Objective For the purpose of finding new bioactive compounds from natural resources, the phytochemical investigation on the fruiting bodies of Amauroderma rude was carried out. Methods The chemical constituents from A. rude were isolated by various technologies, such as silica gel, Sephadex LH-20, MCI-gel resin, and high performance liquid chromatography. The isolated compounds were elucidated by spectroscopic methods, including extensive 1D, 2D NMR, and ESI-MS techniques. Results Three compounds (1-3) including a new norlignan, were isolated from this mushroom. Their structures were identified as 4-ethyoyl-E-3-(3,4-dihydroxybenzylidene)-5-(3,4-dihydroxyphenyl)furan-2-one (1), esculetin (2), and caffeic acid (3). Conclusion Compound 1 is a new compound isolated from this fungus, which is identified as amauroderin A.
ABSTRACT
AIM@#To study the chemical constituents and bioactivity of the seeds of Crataegus pinnatifida.@*METHODS@#The chemical constituents were isolated and purified by macroporous adsorptive resin D101, silica gel, and ODS column chromatography, and preparative HPLC. Their structures were elucidated on the basis of spectroscopic methods. In addition, the cytotoxic activities of compounds 1-4 were investigated on OPM2 and RPMI-8226 cells.@*RESULTS@#Four compounds were obtained and their structures were identified as (7S, 8S)-4-[2-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-1-(hydroxymethyl)ethoxy]-3, 5-dimethoxybenzaldehyde (1), (+)-balanophonin (2), erythro-guaiacylglycerol-β-coniferyl aldehyde ether (3), buddlenol A (4).@*CONCLUSION@#Compound 1 is a novel norlignan, while compounds 1-4 exhibited marginal inhibition on the proliferation of OPM2 and RPMI-8226 cells.